Maastricht University – Human biology, Movement Sciences

Maastricht University – Human biology, Movement Sciences

PO Box 616, 6200 MD Maastricht
Universiteitssingel 50, 6229 ER Maastricht, NL

Contact
Luc van Loon
L.vanLoon@maastrichtuniversity.nl
31(0)433881397 /31(0)613260552

Ellen Blaak
e.blaak@maastrichtuniversity.nl

http://www.m3-research.nl
http://www.sirc.unimaas.nl

Research line
Health and Disease

Topics
protein, carbohydrate and fat and metabolism in vivo in humans
(nutritional) intervention studies
to assess substrate fluxes in different physiological situations and/or patient groups.

Isotopes
¹³C, ¹⁵N, ²H

Instruments
GC/C/IRMS, GC/MS

·        Thermo Scientific MAT-253 GC combustion IRMS
·        Agilent GC-MS (GC:7870A; MS:5975C) (Electron Impact mode)
·        Agilent GC-MS (GC: 6890N; MS: 5973) (EI- and CI-mode)
·        Finnigan DELTAplus GC combustion IRMS with detectors for CO₂ and N₂.

GC-C-IRMS:
·        Enrichment of breath CO₂
·        Concentration and enrichment of CO2 in blood samples
·        Deuterated water enrichment
·        Enrichment of ¹³C-labeled glucose and fatty acids in plasma samples and muscle     extracts
·        Enrichment of ¹³C-labeled fatty acids in plasma FFA and plasma triglycerides and in the different lipid fractions of muscle
·        Enrichment of ¹³C- and ¹⁵N-labeled amino acids in plasma samples, in mixed muscle protein and albumin samples.

GC-MS:
·        Plasma enrichment of [²H₅]glycerol, [U-¹³C]glycerol, [1-¹³C]KIC, [6,6-²H₂]glucose,
[²H₂]palmitate (enrichment in plasma FFA and triglycerides), [²H5-ring]phenylalanine, [1-¹³C]phenylalanine, [1-¹³C]leucine,
[1-¹³C]tyrosine [2H₂]tyrosine and [2H₄]tyrosine,
·        Concentrations of phenylalanine, tyrosine and leucine
·        [²H₂]tyrosine
·        [U¹³C]phenylalanine
·        [²H₅]phenylalanine
·        Intrinsically [1-¹³C]phenylalanine and [1-¹³C]leucine labeled milk protein
·        [²H₂]glucose
·        [U¹³C]glucose
·        [²H₂]palmitate
·        [U¹³C]palmitate
·        [²H₅]-glycerol